Target (protein/gene name): NADH-quinone oxidoreductase subunit D NCBI Gene # or RefSeq#:3266845 Protein ID (NP or XP #) or Wolbachia#: Q5GTF8 Organism: Wolbachia Etiologic Risk Group (see link below): N/A Background/Disease Information: Essentiality of this protein: http://tdrtargets.org/targets/view?gene_id=232711 Complex of enzymes: EC#:1.6.5.3
Structure Available (PDB or Homology model) -- PDB # or closest PDB entry if using homology model: -- Query Coverage (if not direct match): -- Max Ident (if not direct match): Druggable Target (see Databases for this): http://tdrtargets.org/targets/view?gene_id=232711 Current Inhibitors: Expression Information (has it been expressed in bacterial cells): Purification Method: Image of protein (PyMol or etc): Amino Acid Sequence: length of your protein in Amino Acids Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website Molar Extinction coefficient of your protein at 280 nm wavelength: CDS Gene Sequence: http://www.uniprot.org/uniprot/Q5GTF8
GC% Content for gene: CDS Gene Sequence (codon optimized) - after having done Primer Design: GC% Content for gene (codon optimized):
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):
Primer design results for 'tail' primers (this is just 2 sequences):
NCBI Gene # or RefSeq#: 3266845
Protein ID (NP or XP #) or Wolbachia#: Q5GTF8
Organism: Wolbachia
Etiologic Risk Group (see link below): N/A
Background/Disease Information:
Essentiality of this protein:
http://tdrtargets.org/targets/view?gene_id=232711
Complex of enzymes:
EC#: 1.6.5.3
Link to BRENDA EC# page: http://www.brenda-enzymes.org/php/result_flat.php4?ecno=1.6.5.3
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents): There is no enzyme assay information see above link
-- link to Sigma (or other company) page for assay or assay reagents (substrates)
see above
-- link to paper that contains assay information
http://tdrtargets.org/targets/view?gene_id=232711
Structure Available (PDB or Homology model)
-- PDB # or closest PDB entry if using homology model:
-- Query Coverage (if not direct match):
-- Max Ident (if not direct match):
Druggable Target (see Databases for this):
http://tdrtargets.org/targets/view?gene_id=232711
Current Inhibitors:
Expression Information (has it been expressed in bacterial cells):
Purification Method:
Image of protein (PyMol or etc):
Amino Acid Sequence:
length of your protein in Amino Acids
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website
Molar Extinction coefficient of your protein at 280 nm wavelength:
CDS Gene Sequence:
http://www.uniprot.org/uniprot/Q5GTF8
GC% Content for gene:
CDS Gene Sequence (codon optimized) - after having done Primer Design:
GC% Content for gene (codon optimized):
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):
Primer design results for 'tail' primers (this is just 2 sequences):